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KMID : 1140120110160040326
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Cancer Prevention Research
2011 Volume.16 No. 4 p.326 ~ p.332
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Alpinia katsumadai Suppresses Migration and 2-O-tetradecanoylphorbol-13-acetate-induced Invasion of HT-1080 Cells through Suppression of Transglutaminase-2, Matrix Metalloproteinase-2, and Matrix Metalloproteinase-9 Expression
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Park Mi-Kyung
Lee Chang-Hoon
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Abstract
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The cause of death of the majority of cancer patients is the development of metastatic lesions at sites distant from the primary tumor. We found extracts of Alpinia katsumadai (AKE) to have anti-migratory and anti-invasion activity. Migration and invasion of HT-1080 cells were measured by using multiwell chambers. Zymography and western blots were used to examine the effect of AKE on expression of matrix metalloproteinases (MMPs) and transglutaminase-2 (tgase-2). AKE dose-dependently suppressed the migration and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced invasion of HT-1080 sarcoma cells. AKE did not show antiproliferative effects on 3 hr pretreatment but 24 hr incubation revealed partly cytotoxic effects of AKE on HT-1080 cells. AKE suppressed the expressions of tgase-2, MMP-2, and MMP-9 in HT-1080 cells as well as suppressed MMP-9 activity. These results suggest that migration and invasion of HT-1080 cells are inhibited by AKE via suppressions of tgase-2, MMP-2, and MMP-9 and antiproliferative activity.
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KEYWORD
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Alpinia katsumadai, HT-1080, Invasion, Migration, MMP-2, 9, Transglutaminase-2
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